Phytochemical Assay and Antioxidant Activity against DPPH of Ethanol Extract from Kenaf Leaf (Hibiscus cannabinus L.)
DOI:
https://doi.org/10.25026/jtpc.v4i5.202Keywords:
kenaf, flavanoid, tannin, saponin, antioxidantsAbstract
Kenaf (Hibiscus cannabinus L.) is an environmentally friendly natural fiber-producing plant that can produce diversified products, such as paper, wallcover, car interior, geotextile, soil safer, fiber drain, particle board, and plastic reinforcement as well as biofuel industry raw materials. Kenaf plant is a plant that has been only part of the fibers of the stem alone so that many unprofitable side products such as kenaf leaves after harvesting only as a waste. In Africa the leaves of kenaf are widely used as medicine This study aims to analyze phytochemical compounds and study the antioxidant activity of the kenaf leaf. The study started by doing phytochemical test and analyzing antioxidant in kenaf leaf and then testing its antioxidant activity with DPPH method. The results showed that the leaves of kenaf contain secondary metabolites of phytochemical compounds such as flavonoids, alkaloids, steroids, saponins, carbohydrates and tannins and have antioxidant activity that is quite good that is 44.4813 ppm.
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(2) Sies, H. 1997. Oxidative Stress: Oxidants and Antioxidants. Experimental Physiology. 82: 291-295.
(3) Soares, J.R., Dinis, T. C. P., Cunha, A. P., Almeida, L. M. 1997. Antioxidant Activities of Some Extracts of Thymus zigis. Free Radical Biology and Medicine. 26: 468-478.
(4) Sunarni, T., Pramono, S., & Asmah, R. 2007. Flavonoids Antioxidants are radical catchers of Kepel leaves (Stelechocarpus burahol (Bl.) Hook f. & Th.). Pharmaceutical Magazine Indonesia. 18 (3): 111-116.
(5) Silalahi, R. M. 2010. Characterization of Simplicia, Phytochemical Screening and Antioxidant Activity Test of Ethanol Extract Leaves of Broccoli Fruit Flower Fraction (Brassicaoleracea L. var. Botrytis L.) [Thesis]. FMIPA Universitas Sumatera Utara, Medan.
(6) Nurnasari, E. 2017. Use of Kenaf leaf and Seed for Health. WARTA [research and development of Tanman Industri, VOl. 23. No.I. Agricultural Research and Development Center. Center for Plantation Research and Development. Bogor.
(7) Harbone, J. B., 1996. Phytochemical Method: Guide of Modern Way to Analyze Plants, Second Issue. Institut Teknologi Bandung, Bandung.
(8) Pratt, D.E. (1992). Natural Antioxidants from Plant material. In Phenolic Compounds in Food and their Effects on Health (Vol II). Antioxidants and Cancer Prevention; Huang, M-T., Ho, C- T., Lee, C., Eds .: ACS Symposium Series 507; American Chemical Society, Washington DC.
(9) Molyneux, P. 2004. The Use of the Stable Free Radical diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Journal of Science of Technology. 26 (2): 211-219.
(10) Cheng Y. and Prusoff W.H., 1973, Relationship Between the Inhibition Constant and the Concentration of Inhibition Which Causes 50 percent Inhibition (IC50) of An 60nzymatic Reaction. Biochemical Pharmacology. 22(23): 3099–3108.
(11) Blois, MS. 2005. Antioxidant determination by the use of stable free radical. Nature. 181: 1191-1200.
(12) Rininta, N. 2008. KLT Autography-CUPRAC as a Quick Detection Technique Antioxidant Compound. [Essay]. FMIPA IPB, Bogor.
(13) Parwata, I.M.O.A., Wiwik, S.R., and Raditya, Y. 2009. Isolation and Antiradical Test of Free Essential Oil in Betel Leaf (Piper betle L.) by Ultraviolet Spectroscopy - Appear. Journal of Chemistry. 3 (1): 7 -