Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume)

Authors

  • Rolan Rusli Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia http://orcid.org/0000-0003-1264-5933
  • Myra Puspha Hardina Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Fairul Muflihah Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia
  • Agung Rahmadani Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

DOI:

https://doi.org/10.25026/jtpc.v3i2.98

Keywords:

n-hexane fraction of Libo leaves (Ficus variegata Blume.), chromatography, antioxidant, antibacterial

Abstract

Research about chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves (Ficus variegata Blume.) has been done in order to know the form of chromatographic profile of antioxidant and antibacterial compounds in n-hexane fraction of Libo leaves. Extraction was done by maceration using methanol. Isolation was done by 2 methods, vacuum liquid chromatography (VLC) then followed by conventional column chromatography. Eluent used are n-hexane-ethyl acetate and methanol-chloroform. The results based on conventional column chromatography are as many as 10 fractions. The fractions are being conducted then by a qualitative testing of the antioxidant activity using DPPH method and antibacterial activity test using bioautography TLC method. The results formed as a chromatographic profile of fraction that contain antioxidant and antibacterial activity, which are as many as 5 fractions (NHB1, NHB3, NHB4, NHC1, NHC3) active as an antioxidant and 4 fractions (NHB1, NHC1, NHC2, NHC3) which are active as an antibacterial. The secondary metabolite contents of the fractions are alkaloid, flavonoid and steroid/terpenoid.

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References

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Published

2015-12-31

How to Cite

Rusli, R., Hardina, M. P., Muflihah, F., & Rahmadani, A. (2015). Profil Kromatografi Senyawa Aktif Antioksidan dan Antibakteri Fraksi N-Heksana Daun Libo (Ficus variegata Blume). Journal of Tropical Pharmacy and Chemistry, 3(2), 124–130. https://doi.org/10.25026/jtpc.v3i2.98

Issue

Vol. 3 No. 2 (2015): J. Trop. Pharm. Chem.

Section

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