Synergistic Effect of Pericarp of Mangosteen and Propolis from Stingless Bee Extracts on Nitric Oxide Scavenging Activity
DOI:
https://doi.org/10.25026/jtpc.v7i1.458Keywords:
synergistic effect, nitric oxide scavenging activity, phytochemical constituents, stingless bee propolis, mangosteen pericarpAbstract
The aim of this research is to study the synergistic effect on the nitric oxide scavenging activity of mangosteen pericarp and the stingless bee (Tetragonula laviceps) propolis extracts and their phytochemical constituents. The propolis and mangosteen pericarp were extracted by reflux method with ethanol. TPC and TFC of propolis extract were 123.73±2.80 mg GAE/g extract and 70.65±11.21 mg QE/g extract, respectively, and mangosteen pericarp extract was 387.93±15.10 mg GAE/g extract and 87.00±5.06 mg QE/g extract, respectively. The ESI-LC-MS data displayed that both extracts have a variety of phytochemical constituents, such as xanthones, flavonoids, and miscellaneous. The synergistic effect of Nitric oxide scavenging activities of propolis and mangosteen pericarp extracts showed higher activity than individual extracts with various concentrations. Thus, the synergistic effect of propolis and mangosteen pericarp extracts may be an alternative source of inflammatory drug development in the future.
Downloads
References
Meechai, I.; Chelong, I. Influence of Harvesting Times on the Total Flavonoid Content and Antiradical Activity of Stingless Bee Propolis. King Mongkut's Agricultural Journal 2020, 38(3): 363 - 369.
Ng, R.F.L.; Abidin, N.Z.; Shuib, A.S.; Ali, D.A.I. Inhibition of nitric oxide production by Solanum melongena and Solanum macrocarpon on RAW 264.7 cells. Frontiers in Life Science 2015, 8(3): 241-248. https://doi.org/10.1080/21553769.2015.1051241
Makchuchit, S.; Itharat, A.; Tewtrakul, S. Antioxidant and nitric oxide inhibition activities of Thai medicinal plants. The Journal of Medical Association of Thailand 2010, 93(suppl.7): s227-s235.
Adebayo, S.A.; Ondua, M.; Shai, L.J.; Lebelo, S.L. Inhibition of nitric oxide production and free radical scavenging activities of four South African medicinal plants. Journal of Inflammation Research 2019, 12: 195-203. https://doi.org/10.2147/JIR.S199377
Nintasen, R. Nitric oxide and atherosclerosis. Huachiew Chalermprakiet Science and Technology Journal 2016, 2(1): 71-79.
Rao, U.S.M.; Ahmad, B.A.; Mohd, K.S. In vitro nitric oxide scavenging and anti-inflammatory activities of different solvent extracts of various parts of Musa paradisiaca. Malaysian Journal of Analytical Sciences 2016, 20(5): 1191-1202. http://dx.doi.org/10.17576/mjas-2016-2005-26
Panche, A.N.; Diwan, A.D.; Chandra, S.R. Flavonoids: an overview. Journal of Nutritional Science 2016, 5(e47): 1-15. https://doi.org/10.1017/jns.2016.41
Manasathien, J.; Khanema, P. Antioxidant and Cytotoxic Activities of Mangosteen Garcinia mangostana Pericarp Extracts. KKU Research Journal 2015, 20(4), 381-392.
Widowati1, W.; Darsono, L.; Suherman, J.; Fauziah, N.; Maesaroh, M.; Erawijantari, P.P. Anti-inflammatory Effect of Mangosteen (Garcinia mangostana L.) Peel Extract and its Compounds in LPS-induced RAW264.7 Cells. Natural Product Sciences 2016, 22(3): 147-153. https://doi.org/10.20307/nps.2016.22.3.147
Dutra, R.P.; Abreu, B.V.B.; Cunha, M.S.; Batista, M.C.A.; Torres, L.M.B.; Nascimento, F.R.F.; Ribeiro, M.N.S.; Guerra, R.N.M. Phenolic Acids, Hydrolyzable Tannins, and Antioxidant Activity of Geopropolis from the Stingless Bee Melipona fasciculata Smith. Journal of Agricultural and Food Chemistry 2014, 62(12): 2549-2557. https://doi.org/10.1021/jf404875v
Ibrahim, N.; Mohd Niza, N.F.S; Mohd Rodi, M.M.; Zakaria, A.J.; Ismail, Z.; Mohd, K.S. Chemical and biological analyses of Malaysia stingless bee propolis extracts. Malaysian Journal of Analytical Sciences 2016, 20(2): 413-422. http://dx.doi.org/10.17576/mjas-2016-2002-26
Pahlavani, N.; Malekahmadi, M.; Firouzi, S.; Rostami, D.; Sedaghat, A.; Moghaddam, A.B.; Ferns, G.A.; Navashenaq, J.G.; Reazvani, R. Safarian, M.; Ghayour-Mobarhan, M. Molecular and cellular mechanisms of the effects of Propolis in inflammation, oxidative stress and glycemic control in chronic diseases. Pahlavani et al. Nutrition & Metabolism 2020, 17(65): 1-12. https://doi.org/10.1186/s12986-020-00485-5
Suttirak, W.; Manurakchinakorn, S. In vitro antioxidant properties of mangosteen peel extract. Journal of Food Science and Technology 2014, 51(12): 3546-3558. http://dx.doi.org/10.1007/s13197-012-0887-5
Roell, K.R.; Reif, D.M.; Motsinger-Reif, A.A. An Introduction to Terminology and Methodology of Chemical Synergy—Perspectives from Across Disciplines. Frontiers in Pharmacology 2017, 28: 158. https://doi.org/10.3389/fphar.2017.00158
Li, X.; Qin, G.; Yang, Q.; Chen, L.; Xie, L. Biomolecular Network-Based Synergistic Drug Combination Discovery. BioMed Research International 2016, 2016: 1-11. https://doi.org/10.1155/2016/8518945
Singchai, B.; Khojulchun, J.; Duangthong, P. Phytochemicals and Biological Activities of Toddalia asiatica Tea. Thai Science and Technology Journal 2017, 25(5): 830-838.
Padalia, H.; Poptani, R.; Chanda, S. Evaluation of in vitro antioxidant properties of solvent extracts of selected medicinal plants and their synergistic efficacy. Journal of Herbs, Spices & Medicinal Plants 2018, 24(8): 15-27. https://doi.org/10.1080/10496475.2017.1357159
Mouhoubi-Tafinine, Z.; Ouchemoukh, S.; Tamendjari, A. Antioxydant activity of some algerian honey and propolis. Industrial Crops and Products 2016, 88: 85-90. https://doi.org/10.1016/j.indcrop.2016.02.033
Asem, N.; Gapar, N.A.A.; Hapit, N.H.A.; Omar, E.A. Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract. Journal of Apicultural Research 2019, 59(4): 1-6. https://doi.org/10.1080/00218839.2019.1684050
Meechai, I.; Phupong, W.; Chunglok, W.; Meepowpan, P. Antiradical activities of xanthones and flavonoids from Garcinia schomburgkiana. International Journal of Pharmacy and Pharmaceutical Sciences 2016, 8(9): 235-238. https://doi.org/10.22159/ijpps.2016v8i9.13213
Afsar, T.; Razak, S.; Shabbir, M.; Khan, M.R. Antioxidant activity of polyphenolic compounds isolated from ethyl-acetate fraction of Acacia hydaspica R. Parker. Chemistry Central Journal 2018, 12(5): 1-13. https://dx.doi.org/10.1186/s13065-018-0373-x
Proença, C.; Albuquerque, H.M.T.; Ribeiro, D.; Freitas, M.; Santos, C.M.M.; Silva, A.M.S.; Fernandes, E. Novel chromone and xanthone derivatives: Synthesis and ROS/RNS scavenging activities. European Journal of Medicinal Chemistry 2016, 115: 381-392. https://doi.org/10.1016/j.ejmech.2016.03.043
Boora, F.; Chirisa, E.; Mukanganyama, S. Evaluation of Nitrite Radical Scavenging Properties of Selected Zimbabwean Plant Extracts and Their Phytoconstituents. Journal of Food Processing 2014, 2014: 1-7. https://doi.org/10.1155/2014/918018
Downloads
Additional Files
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Journal of Tropical Pharmacy and Chemistry
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
